molecular biology, cellular biology, biochemistry, microscopy
WHAT WE DO
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This FIRE stream investigates how bacterial translational fidelity and ribosome dynamics drive phenotypic heterogeneity and antibiotic tolerance at the single-cell level. Using E.coli as a model and eventually Salmonella, our primary research goal is to map the metabolic and regulatory networks that allow bacterial populations to survive stress. We specifically aim to dissect mechanisms like stop-codon readthrough, characterize ribosome allocation during starvation, and identify novel metabolic regulators.
WHY IT MATTERS
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Non-genetic antibiotic tolerance remains a major hurdle in clinical treatments. By linking fundamental ribosome biogenesis to pathogenic outcomes, this work addresses critical, unanswered questions in bacterial stress physiology and host-pathogen interactions. From a practical perspective, this agenda is perfectly structured for iterative student discovery. Undergraduates will use a versatile toolkit, including high-throughput genetic screens, fluorescence microscopy, and single-cell quantitative imaging. This design allows students to safely test original hypotheses, troubleshoot unexpected phenotypes, and generate reproducible data that advances molecular microbiology.
WHAT YOU LEARN
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To investigate bacterial phenotypic heterogeneity and translational fidelity, students will master a progressive set of laboratory techniques.
Foundational Microbiology and Molecular Biology
Students will begin with essential skills, including aseptic techniques and microbial culture maintenance. They will also learn core genetic manipulation methods such as RNA and DNA extraction, primer design, PCR, and targeted gene disruption.
Advanced Single-Cell Imaging
To study single-cell dynamics, students will be trained in fluorescence microscopy and single bacteria quantification. These imaging techniques will allow them to visually observe phenotypic variations within identical bacterial populations.
Biochemical and High-Throughput Assays
To investigate how bacteria adapt to antibiotic stress, students will perform time-kill kinetics and conduct high-throughput genetic screening to characterize novel metabolic regulators of antibiotic tolerance and survival.
These methods will be introduced iteratively. Students will actively troubleshoot protocols and refine their experimental designs when facing unexpected results, fostering genuine independence and scientific resilience.
Related Resources
More information soon.
Dr. Zhihui Lyu
Assistant Clinical Professor
Dr. Jiqiang (Lanny) Ling
Faculty Collaborator